Comparative digital cartilage histology for human and common osteoarthritis models
Comparative digital cartilage histology for human and common osteoarthritis models
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Douglas R Pedersen, Jessica E Goetz, Gail L Kurriger, James A MartinDepartment of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, USAPurpose: This study addresses the species-specific and site-specific details of weight-bearing articular cartilage zone depths and chondrocyte distributions among humans and common osteoarthritis (OA) animal models using contemporary digital imaging tools.Histological analysis is the gold-standard research tool for evaluating cartilage health, OA severity, and treatment efficacy.Historically, evaluations were made by expert analysts.However, state-of-the-art tools have been developed that allow for digitization of entire histological sections for computer-aided analysis.Large volumes of common digital cartilage metrics directly complement elucidation of trends in OA inducement and concomitant potential treatments.
Materials and methods: Sixteen fresh human knees, 26 adult New Zealand rabbit stifles, and 104 bovine lateral plateaus were measured for four cartilage zones and nightstick twm-850xl the cell densities within each zone.Each knee was divided into four weight-bearing sites: the medial and lateral plateaus and femoral condyles.Results: One-way analysis of variance followed by pairwise multiple comparisons (Holm–Sidak read more method at a significance of 0.05) clearly confirmed the variability between cartilage depths at each site, between sites in the same species, and between weight-bearing articular cartilage definitions in different species.Conclusion: The present study clearly demonstrates multisite, multispecies differences in normal weight-bearing articular cartilage, which can be objectively quantified by a common digital histology imaging technique.
The clear site-specific differences in normal cartilage must be taken into consideration when characterizing the pathoetiology of OA models.Together, these provide a path to consistently analyze the volume and variety of histologic slides necessarily generated by studies of OA progression and potential treatments in different species.Keywords: knee, osteoarthritis, imaging, rabbit, bovine, cell density.